|
KMID : 0380420010250030185
|
|
Journal of Prventive Veterinary Medicine
2001 Volume.25 No. 3 p.185 ~ p.192
|
|
|
Experimental Inoculation of Brucella abortus Strain RB51 in Pregnant Dogs
|
|
Kiku, Matsuda
|
|
|
Abstract
|
|
|
|
Brucella abortus strain RB51 (SRB51) is an approved brucellosis vaccine in cattle. This study was caried out to know the immunological responses and abortion status in dogs inoculated with SRB51. Six pregnant dogs were subcutaneously inoculated with 1.0×109 CFU of SRB51. The animals were monitored at regular intervals by the standard tube agglutination test (STAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and dot-blot. Using the whole cell antigen of SRB51, the mean antibody titer against SRB51 was below 1:10 at prior to inoculation in dot-blot. The titer gradually increased up to 1:370 by the 3rd week post-inoculation. In STAT and RBT, using the whole cell antigen of B. abortus 1119-3, and 2ME-RSAT, using the whole cell antigen of B. canis M－, the sera against SRB51 did not react with both antigens. SRB51 was not isolated from the blood of dogs subcutaneously inoculated with the bacteria. In addition, subcutaneous inoculation of SRB51 did not lead to abortion in pregnant dogs.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|